Dr. Stephen Stack Professor  Department of Biology sstack@lamar.colostate.edu

Several studies are underway. 1) a feasibility study for preparing a map of recombination nodules (RNs) on maize synaptonemal complexes (similar to the map we have produced for tomato). 2) In situ hybridization to locate single copy sequences on tomato synaptonemal complexes (SCs). 3) RAD51 protein is thought to be involved in crossing over. Antibodies are being used for immunolocalization of RAD51 protein at the electron microscope level in primary microsporocytes of tomato. 4) Identification and isolation of synaptonemal complex and recombination nodule proteins from Lilium longiflorum is important. 5) Another goal is to identify genes for SC and RN proteins in maize and then utilize Pioneer Hybrid's system to obtain mutants for the genes so their function can be determined.