SECTION I - INFECTIOUS AGENT


NAME: Epidermophyton floccosum, Microsporum spp., Trichophyton spp.

SYNONYM OR CROSS REFERENCE: Dermatophytes, Dermatomycosis, Tinea, Ringworm, Epidermophytosis, Trichophytosis, Microsporosis.

CHARACTERISTICS: Form hyphae and arthrospores in keratinized tissue, develop characteristic colonies and conidia in culture, sexual spores in some species.

SECTION II - HEALTH HAZARD

PATHOGENICITY: Mycotic disease of keratinized areas of the body (hair, skin and nails) and of variable severity depending on genera and species of dermatophyte; usually scaling or hair loss or breakage, sometimes erythema, induration, crusting or suppuration; lesions often circular or annular because of central healing.

EPIDEMIOLOGY: Worldwide, relatively frequent; more common in hot, humid climates with crowding or debilitation; season prevalence varies with age.

HOST RANGE: Humans, domestic and wild animals (depending on genera and species of dermatophyte - host specific).

INFECTIOUS DOSE: Unknown.

MODE OF TRANSMISSION: Contact of minor traumatized skin site with infected individual or fomites contaminated with desquamated epithelium.

INCUBATION PERIOD: 5-12 days.

COMMUNICABILITY: Communicable as long as infective lesions are present and viable.

SECTION III - DISSEMINATION

RESERVOIR: Humans (anthropophilic); animals (zoophilic); soil (geophilic).

ZOONOSIS: Yes; farmers, livestock handlers, laboratory workers, and persons who milk infected animals are at greater risk.

VECTORS: None

SECTION IV - VIABILITY

DRUG SUSCEPTIBILITY: Griseofulvin, miconazole, clotrimazole - depending on agent.

SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to 1% sodium hypochlorite (20% commercial bleach), phenolics, glutaraldehyde, formaldehyde; susceptibility to 70% ethanol questionable.

PHYSICAL INACTIVATION: Inactivated by moist heat (121oC for at least 15 min).


SURVIVAL OUTSIDE HOST: Agents survive for months to years in dry, cool, shaded areas.

SECTION V - MEDICAL

SURVEILLANCE: Monitor for symptoms; confirm by microscopic examination of scrapings.

FIRST AID/TREATMENT: Bathing with soap and water and topical application of appropriate fungicide, griseofulvin for scalp involvement and for infections unresponsive to topical agents.

IMMUNIZATION:
None

PROPHYLAXIS: None

SECTION VI - LABORATORY HAZARDS

LABORATORY-ACQUIRED INFECTIONS: Sixth most commonly reported lab-acquired infection; 161 reported cases most frequently associated with T. mentagrophytes; most acquired through contacts with naturally or experimentally infected laboratory animals (mice, rabbits, guinea pigs) and rarely, with handling cultures.

SOURCES/SPECIMENS: Skin, hair, and nails of human and animal hosts.


PRIMARY HAZARDS: Contact with infected laboratory animals with inapparent or apparent infections.


SPECIAL HAZARDS: None


SECTION VII - RECOMMENDED PRECAUTIONS

CONTAINMENT REQUIREMENTS: Biosafety level 2 practices, containment equipment and facilities for all laboratory and experimental animal activities with dermatophytes.

PROTECTIVE CLOTHING: Laboratory coat; gloves when handling infected materials.

OTHER PRECAUTIONS: Good sanitation, cleaning and disinfection important.

SECTION VIII - HANDLING INFORMATION

SPILLS: Allow aerosols to settle; wearing protective clothing, gently cover spill with paper towels and apply sodium hypochlorite, starting at perimeter and working towards the center; allow sufficient contact time (30 min) before clean up.

DISPOSAL: Decontaminate before disposal; steam sterilization, incineration, chemical disinfection.

STORAGE: In sealed containers that are appropriately labeled.

Date prepared: October 11, 1997 Prepared by: Canadian Office of Biosafety Information edited by the Colorado State University Office of Biosafety; June 16, 1998.

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