SECTION I - INFECTIOUS AGENT


NAME: Colorado tick fever virus

SYNONYM OR CROSS REFERENCE: Tick-borne fever, arbovirus

CHARACTERISTICS: Reoviridae, coltivirus, 65-70mn diameter, ds RNA (Note change from single to double strand RNA)

SECTION II - HEALTH HAZARD

PATHOGENICITY: Acute febrile, often diphasic, dengue-like disease with infrequent rash; brief remission followed by second bout of fever lasting 2-3 days; neutropenia, thrombocytopenia; occasional encephalitis, myocarditis, or tendency to bleed; deaths are rare.

EPIDEMIOLOGY: Western North America; most frequent in adult males, but also affects children and women; seasonal incidence parallels the period of greatest tick activity; endemic in occurrence and common in affected area.


HOST RANGE: Humans, small mammals.


INFECTIOUS DOSE: Unknown.


MODE OF TRANSMISSION: By bite of an infective vector tick; immature ticks acquire infection by feeding on infected viremic animals; ticks remain infected through the various molts and transmit virus to man by feeding as adult ticks.


INCUBATION PERIOD: Usually 4-5 days.


COMMUNICABILITY: Not directly transmitted from person to person, except by transfusion; ticks remain infective for life; virus is present in humans during the course of fever, from 2-16 weeks or more after onset.

SECTION III - DISSEMINATION

RESERVOIR: Small mammals, ground squirrels, chipmunk, porcupine.


ZOONOSIS: Yes, through bite of an infected tick.


VECTORS: Tick - Dermacentor andersoni.

SECTION IV - VIABILITY

DRUG SUSCEPTIBILITY: N/A


SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to 1% sodium hypochlorite (20% commercial beach), 70% ethanol, glutaraldehyde, formaldehyde.


PHYSICAL INACTIVATION: Inactivated by heat (50-60o C for at least 30 min.).


SURVIVAL OUTSIDE HOST: Does not survive out of host.

SECTION V - MEDICAL

SURVEILLANCE: Serological studies or isolation of virus from blood.


FIRST AID/TREATMENT: No specific treatment.


IMMUNIZATION: None available.


PROPHYLAXIS: None available.

SECTION VI - LABORATORY HAZARDS

LABORATORY-ACQUIRED INFECTIONS: 16 reported laboratory acquired infections.


SOURCES/SPECIMENS: Blood, CSF, other tissues; arthropods, depending on the stage of infection.


PRIMARY HAZARDS: Accidental parenteral inoculation, contact of the virus with broken skin or mucous membranes, bites of infected laboratory rodents or arthropods; infectious aerosols may also be a potential source of infection.


SPECIAL HAZARDS: None

SECTION VII - RECOMMENDED PRECAUTIONS

CONTAINMENT REQUIREMENTS: Biosafety level 2 practices, safety equipment and facilities are recommended for activities with potentially infectious clinical materials and arthropods and for manipulations of infected tissue cultures, embryonated eggs, and rodents.


PROTECTIVE CLOTHING: Laboratory coat; gloves and gown when working with infectious materials.


OTHER PRECAUTIONS: None

SECTION VIII - HANDLING INFORMATION

SPILLS: Allow aerosols to settle; wearing protective clothing, gently cover spill with paper towel and apply 1% sodium hypochlorite (20% commercial bleach), starting at perimeter and working towards the center- allow sufficient contact time before clean up (30 min).


DISPOSAL: Decontaminate before disposal; steam sterilization, incineration.


STORAGE: In sealed containers that are appropriately labeled.


Date prepared: September 1996 Prepared by: Canadian Office of Biosafety.
Information edited by the Colorado State University Office of Biosafety; June 16, 1998.

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