SECTION I - INFECTIOUS AGENT


NAME: Toxocara canis, T. cati

SYNONYM OR CROSS REFERENCE: Toxocariasis, visceral larva migrans, larva migrans visceralis, ocular larva migrans.

CHARACTERISTICS: Enteric nematode, helminth, larvae are 400 um X 20 um; adults females are 5 to 18 cm long and adult males 4 to 10 cm long; eggs are about 85 um X 75 um with a thick brown shell.

SECTION II - HEALTH HAZARD

PATHOGENICITY: Two distinct larva migrans infections can occur: visceral larva and ocular larva migrans, both due to the systemic migration of the larval form of the helminth; visceral larva migrans is characterized by hepatosplenomegaly, fever, and eosinophilia, symptoms depends on the site and extent of larval migration; ocular larva migrans symptoms include leukokoria, loss of vision in the affected eye, eye pain and strabismus. Disease is rarely fatal.

EPIDEMIOLOGY: Worldwide; eggs are ubiquitous in the soil wherever dogs and cats defecate; common in preschool children with a history of dirt-eating; ocular disease more common in school-age children, increasingly recognized in humans.

HOST RANGE: Humans, dogs and cats.

INFECTIOUS DOSE: Not known

MODE OF TRANSMISSION: By direct or indirect transmission of eggs from contaminated soil to the mouth; direct transfer by eating raw, unwashed vegetables and dirt (children).

INCUBATION PERIOD: Varies from weeks to months depending on the intensity of the infection. Eggs infective after 1-3 weeks incubation.

COMMUNICABILITY: Not directly transmitted from person-to-person.

SECTION III - DISSEMINATION

RESERVOIR: T. canis in dogs; T. cati in cats.

ZOONOSIS: Yes - disease transmitted by contaminated feces of dogs and cats.


VECTORS: None

SECTION IV - VIABILITY

DRUG SUSCEPTIBILITY: Sensitive to diethylcarbamazine, thiabendazole.

SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to 1% sodium hypochlorite (20% commercial bleach), 2% glutaraldehyde.

PHYSICAL INACTIVATION: Eggs are sensitive to drying.


SURVIVAL OUTSIDE HOST: Eggs can remain viable in soil for many months.

SECTION V - MEDICAL

SURVEILLANCE: Monitor for symptoms; confirm by ELISA testing, microscopic demonstration of eggs in tissue samples.

FIRST AID/TREATMENT: Administration of appropriate drug therapy; typically diethylcarbamazine for visceral larva migrans, corticosteroids for ocular larva migrans.

IMMUNIZATION: None available.

PROPHYLAXIS: None available.

SECTION VI - LABORATORY HAZARDS

LABORATORY-ACQUIRED INFECTIONS: None reported to date.

SOURCES/SPECIMENS: Blood and blood products; feces; ocular tissues.

PRIMARY HAZARDS: Ingestion; contamination of mucous membranes; skin penetration of larvae.

SPECIAL HAZARDS: Avoid the generation of aerosols when working with infected laboratory animals; avoid contact with contaminated feces.

SECTION VII - RECOMMENDED PRECAUTIONS

CONTAINMENT REQUIREMENTS: Biosafety level 2 practices and containment for activities involving the infective stage of the parasite, infectious body tissues and fluids.

PROTECTIVE CLOTHING: Laboratory coats; gloves when skin contact with infectious materials is unavoidable.

OTHER PRECAUTIONS: Good personal hygiene and frequent hand washing.

SECTION VIII - HANDLING INFORMATION

SPILLS: Allow aerosols to settle; wearing protective clothing, gently cover spill with absorbent paper towel and apply 1% sodium hypochlorite (20% commercial bleach), starting at the perimeter and working towards the center; allow sufficient contact time (30 min) before clean up.

DISPOSAL: Decontaminate all wastes before disposal; steam sterilization, chemical disinfection, incineration.

STORAGE: In sealed containers that are appropriately labeled.

Date prepared: October 11, 1997 Prepared by: Canadian Office of Biosafety Information edited by the Colorado State University Office of Biosafety; June 16, 1998.

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